A High Performance Continuous Electroporation Chip
نویسندگان
چکیده
Electroporation is an effective method for delivery of biological components into mammalian cells and is of great importance for modern life science. Here we report a novel continuous electroporation chip with great performance. Integration of the microfluid channel, micromachined Au electrodes and hydrodynamic focusing enables high-throughput and high-efficiency electroporation. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent transfection rate (90%), cell viability (60%) and cell treating speed (1.5 10 cells per minute), all much higher than those reported in literature.
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